Yes, absolutely. You may not get an error message but it simply won't work. A different glass material or the windshield not sitting properly can cause refraction problems for the camera. Ford manual is very specific as to what to watch for when replacing a windshield.
I have done a few, just put a drill stop on the bit about a 1/4-3/8" from the tip. Ford has a stud sert that requires a special tool to mount the extended part of the vent hose. You could use a 1/4-20 but sert or something similar.
If you look up TSB 24-2185 it shows and tells you everything you need to know. If you can't find it, let me know and I will try and save a copy from service data and upload it
Good on you for changing those fluids, very important for the life of those units. Add nothing to the RDU fluid. It is specifically formulated for the clutches in those units and need nothing else. There is an improved vent hose for some 21 Sports (TSB). It goes a little higher in the rear...
An effective way to flush without a machine is to disconnect to trans to cooler line from the rad. Connect a hose and put other end in pail. Remove the vent from the top of the trans an inset funnel. Put in a litre of fuel and start car. Car will pump fluid out into pail as you replace it by...